Response Surface Optimization of Gamma Irradiation Synthesis of Alginate-Stabilized Silver Nanoparticles Without Addition of a Hydroxyl Radical Scavenger

The use of isopropanol as a hydroxyl radical scavenger on the radiosynthesis alginate-stabilized silver nanoparticles (AgNPs) can limit its application in nanomedicine. Meanwhile, optimum condition for gamma irradiation synthesis AgNPs without addition has not been reported yet. In this study, optimization process was carried out using response surface methodology (RSM) combined with Central Composite Design (CCD). three processing conditions, i.e. radiation dose, precursor ion concentration, and alginate concentration were selected decision variables to maximize two responses terms conversion yield AgNP responses. results indicated that regression model fit linearly two-factor interaction linear model, respectively. significant effect factor indicates dual stabilizing–scavenging role alginate. conditions derived from CCD-RSM obtained at 20 kGy 7.78 mM 1.2 % (w/v) desirability 0.731. actual experimental 65.43% 480.91 ppm which within prediction interval confidence 95 %. under had spherical shape, 97.4 volume size distribution 6.50-28.21 nm, zeta potential -28.3 mV.